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The General Introduction of Yeast Expression

Yeast expression systems offer several advantages, including easy culture conditions, rapid growth, low cost, post-translational processing, and easy access to soluble active recombinant proteins. Among all yeast strains, Pichia pastoris is particularly notable for its swift growth and efficient secretion and expression capabilities.

Synbio Technologies provides highly efficient yeast expression protein services by leveraging our advanced protein manufacture platform and industry-leading codon optimization software, NGCodon. Our services save both time and cost while delivering high-quality recombinant proteins to meet the diverse needs of our customers.

Highlights

Large-scale Expression
One-stop Service from Genes to Proteins
Free Codon Optimization
Multiple Purification Methods to Meet Different Requirements

Service Details

Steps	Description	Turnaround Time	Price
Gene Synthesis (optional)	• Codon optimization • Gene synthesis • Vector construction (pPICZα, pPIC9, pGAPZα…)	Starting from 10 BD	Quote
Electrotransformation and Screening Positive Clones (PCR method)	• Linearized the plasmid and transformed into pichia pastoris cells to screen positive clones	2-3 weeks	Starting from $3,499
Pilot Protein Expression	• Three positive clones will be used to verify the expression, then perform expression analysis validation (WB)	2-3 weeks
Protein Expression and Purification	• 1 L or larger scale expression • One or more steps purification • Purity will be detected by SDS-PAGE or Western blot (optional)	3-4 weeks

QUOTE

Deliverables

2-5 μg lyophilized plasmid DNA

1 tube of glycerol bacteria/puncture bacteria

Sequencing validation report

Screening report of positive clones

Optimal expression strain

Purified protein products

Certificate of analysis (COA)

FAQs

What types of yeast are commonly used for expression?

Some popular yeast strains used for expression include Saccharomyces cerevisiae (baker's yeast) or Pichia pastoris (methylotrophic yeast), depending on your specific applications

Why do you specifically use Pichia pastoris ?

Pichia pastoris is the preferred choice for the expression of recombinant proteins in yeast due to its rapid growth, abundant commercial expression vectors, and efficient secretion expression.

What are the main advantages of a yeast expression system?

Yeast expression systems combine the advantages of both prokaryotic and higher eukaryotic systems. It is characterized by rapid culture conditions, fast growth, low cost, post-translational processing of proteins, and easy access to soluble active recombinant proteins.

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