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The General Introduction of Hybridoma Antibody Sequencing

Monoclonal antibody sequencing includes hybridoma cell line sequencing and clonal B cell sequencing. Each of these types of cells can produce a single antibody against a unique antigen after screening.

Synbio Technologies uses Sanger sequencing and NGS sequencing technologies to provide both accurate and efficient hybridoma/clonal B cell sequencing services across a variety of species. Through our rich experience in the field of antibody sequencing and utilizing a professional immunogenetics database, we are able to design and sequence antibodies from any species (mouse, rat, human, rabbit, hamster, camel, sheep, etc.) and various antibody subtypes (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, IgE, IgA, etc.).

Highlights

100% Accurate Sequencing
Sequencing of Heavy and Light Variable Regions or Full-Length Antibodies
Design and Sequence Antibodies from Any Species and Subtypes
One-stop Solution for Hybridoma Sequencing and Antibody Production
Time and Cost Effective

Service Details

Method	Price	TAT	Deliverables
Sanger sequencing	Starting from $800	As fast as 5 business days	• Raw sequence data with guaranteed accuracy. • Final sequence report features variable heavy and light chain sequences, or complete DNA and amino acid sequences, including annotated CDR regions. • Plasmid contains antibody coding sequence (upon your request).
NGS	Starting from $2,000	2 – 3 weeks	• Frequency of the sequences identified by NGS. • Final sequence report with variable heavy chain and light chain DNA and amino acid sequence with CDR regions annotated.

* For hybridoma cell lines or clonal B cells, customers will need to submit hybridoma cell samples (>1×10⁶ cells) and indicate the species and subtype of the monoclonal antibody.

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Workflow

Standard Hybridoma Sequencing Process

NGS Sequencing Process

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Deliverables

Sample	Shipping
Cells in RNAlater or DNA/RNA Shield (Recommended)	Ice pack (Domestic) Dry ice (International)
Cell pellets	Dry ice
Cells in cryopreservation media	Dry ice
Good quality total RNA	Dry ice

FAQs

What are the applications of hybridoma sequencing?

Helps identify and define the attributes of antibodies: Monoclonal antibody sequencing can provide valuable insights into the characteristics of the antibody molecule, such as its specificity, binding affinity, and structural elements. These insights can help researchers optimize their monoclonal antibody development and manufacturing processes.

Prevents cell line loss: Monoclonal antibody sequencing can also facilitate the permanent storage of hybridoma cell lines and prevent the loss of valuable monoclonal antibody-producing cells. This is especially crucial for long-term research projects that require consistent access to the same cell lines.

Aids in the selection of appropriate cell lines: Sequencing monoclonal antibodies can help researchers identify the ideal cell lines for the development of recombinant monoclonal antibodies. With this knowledge, researchers can select the best cell line expression systems and generate monoclonal antibodies with higher purity.

What are the advantages of hybridoma cell sequencing over direct sequencing of antibodies based on protein analysis?

Hybridoma sequencing can provide more accurate and reliable results, preventing potential risks in protein sequencing such as the difficulty in distinguishing leucine/isoleucine.

What is the significance of antibody gene sequencing of hybridoma cells

Corresponding sequences can be obtained from hybridoma cells, and when monoclonal antibodies are needed in subsequent projects, recombinant antibodies can be prepared by using the sequences of the genes measured. It solves the problem of cell death or loss of high specificity in the process of hybridoma preservation.

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