The General Introduction of Mammalian Expression
Synbio Technologies' Mammalian Expression Platform provides comprehensive services, including high-throughput
recombinant antibody production, large-scale antibody production, and recombinant protein production ranging milligrams to grams. We deliver recombinant protein products with ≥98% purity and endotoxin levels below 0.001 EU/μg.
Our mammalian cell expression systems offer proper protein folding and complex post-translational modifications, ensuring that the expressed products closely mimic the molecular structure, physicochemical properties, and biological functions of natural higher organism proteins.
Highlights
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- Free Codon Optimization
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- ≥98% Purity
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- ＜0.001 EU/μg Endotoxin
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- High Yield Ranging from μg-g
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- Fast Delivery in 3-4 Weeks
Service Details
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Service
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Description
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Turnaround Time
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Price
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Gene Synthesis (Optional)
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• Codon optimization
• Gene synthesis
• Vector construction
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Starting from 10 BDs
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Quote
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Pilot Protein Expression
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• Cell transient transfection
• Pilot Expression
• Expression analysis validation (WB)
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2-3 weeks
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Large-scale Protein Preparation
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• 200ml, 1 L or larger scale expression
• One or more steps purification
• Purity will be detected by SDS-PAGE or Western blot (Optional)
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2-3 weeks
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*The delivery time of this step may vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.
Workflow
Deliverables
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2-5µg lyophilized plasmid DNA
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1 tube of glycerol bacteria/puncture bacteria
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Sequencing validation report
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Expression evaluation report
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COA
FAQs
1. Post-translational protein modifications are complex and sometimes the cell fails to process them correctly. This results in degradation of the protein
2. Proteins contain special sequences (e.g. highly repetitive sequences), resulting in low or abnormal protein expression
3. Inappropriate expression system
The range of proteins that can be expressed in mammalian cell expression systems is usually between 10 kDa and 150 kDa.
HEK293 Cells
- High transfection efficiency
- Rapid and stable growth
- Human cell line
- Transient expression
CHO Cells
- Scalability
- Various post-translational modifications
- Slow growth
- Transient and stable expression
Resources
Get in Touch with Us
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