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The General Introduction of Custom DNA Oligos

Synbio Technologies’ DNA oligo synthesis platform equips state-of-the-art synthesizers and leading professional teams with vast experience in oligo synthesis. We offer custom DNA oligo synthesis options including but not limited to standard oligos, micro-oligos, large-scale oligos, degenerate primers, long oligos synthesis, etc.

Highlights

High Quality

ISO 9001 & ISO 13485 quality certifications
High Stability

Ensures batch-to-batch consistency and traceability
High Customization

Flexible synthesis scales and multiple alternative purification options
Cost-Effective

Competitive prices and affordable services

Service Details

Synbio Technologies provides a fast and convenient standard DNA oligo synthesis. Large scale and bulk custom DNA oligos can be ordered with identical high purity, competitive prices, and fast turnaround time.

Standard Oligo Synthesis (Price per Base)
Synthesis Scale	10nm	25nm	50 nmol	100 nmol	200 nmol	1 μmol
11-59nt	$0.07	$0.10	$0.15	$0.30	$0.40	$1.00
60-90nt	$0.08	$0.12	$0.18	$0.35	$0.50	$1.20

* Longer oligos, 96/384 plate batch oligos or other large scale synthesis are available, please contact us for a quote.

* Please refer to the purification table for the final output corresponding to different synthesis scale.

Purification (Price per Oligo)
11-59nt	Purification Method	Synthesis Scale	10nm	25nm	50 nmol	100 nmol	200 nmol	1 μmol
	DSL	Final Yield	1 OD / 5 nmol	2 OD / 10 nmol	5 OD / 25 nmol	10 OD / 50 nmol	15 OD / 75 nmol	50 OD / 250 nmol
	DSL	Price	Free	Free	Free	Free	Free	Free
	PAGE Plus	Final Yield	–	–	2 OD / 10 nmol	5 OD / 25 nmol	10 OD / 50 nmol	30 OD / 150 nmol
	PAGE Plus	Price	–	–	$5.00	$5.00	$5.00	$5.00
	PAGE	Final Yield	–	–	–	2 OD / 10 nmol	5 OD / 25 nmol	10 OD / 50 nmol
	PAGE	Price	–	–	–	$15.00	$15.00	$30.00
	HPLC	Final Yield	–	–	–	2 OD / 10 nmol	5 OD / 25 nmol	10 OD / 50 nmol
	HPLC	Price	–	–	–	$10.00	$10.00	$20.00
60-90nt	DSL	Final Yield	1 OD / 1.5 nmol	2 OD / 3 nmol	5 OD / 7.5 nmol	10 OD / 15 nmol	15 OD / 22.5 nmol	50 OD / 75 nmol
	DSL	Price	Free	Free	Free	Free	Free	Free
	PAGE Plus	Final Yield	–	–	2 OD / 3 nmol	5 OD / 7.5 nmol	10 OD / 15 nmol	30 OD / 45 nmol
	PAGE Plus	Price	–	–	$5.00	$5.00	$5.00	$5.00
	PAGE	Final Yield	–	–	–	2 OD / 3 nmol	5 OD / 7.5 nmol	10 OD / 15 nmol
	PAGE	Price	–	–	–	$15.00	$15.00	$30.00
	HPLC	Final Yield	–	–	–	2 OD / 3 nmol	5 OD / 7.5 nmol	10 OD / 15 nmol
	HPLC	Price	–	–	–	$10.00	$10.00	$20.00

* The total price of oligo=price/base*nt + purification price.

* If you need other synthesis scale and lengths, please contact us for a quote.

* For modified oligos please click Oligo Modification & Labeling.

Length	Final Yield	Purification	Turnaround Time/Price	Deliverables
15-90nt, no fewer than 96 oligos	0.1-1 nmol	Desalt or PAGE Plus	Quote	Plate delivery or pool delivery

Synbio Technologies provides mass production of oligos with high purity in measurements from milligrams to grams. These oligos are mainly used in kit development, drug discovery (ASOs drugs), aptamers, DEL (DNA encoded compound library), IVD research fields, etc.

Large-Scale Oligo Synthesis（Unmodified DNA Price）
Final Yield	10–25 nt	26–40 nt	41–55 nt	>55 nt
25 mg	$1,338	$1,451	$1,761	Quote
50 mg	$1,427	$1,524	$1,797
100 mg	$1,587	$1,761	$2,119
200 mg	$1,693	$1,949	$3,208
250 mg	$1,787	$2,042	$2,480
500 mg	$2,383	$2,795	$3,438
1 g	$4,289	$4,714	$5,642

*If you need to know the turnaround time and the price of modified DNA oligos, please contact us for a quote.

Purification	QC Analysis	Deliverables	Modifications and Probes
Purification	QC Analysis	Deliverables	Backbone	Linker	qPCR Probes
HPLC/ PAGE	● HPLC/LC-MS ● Endotoxin Control/Bioburden (optional)	● Lyophilized products ● COA file	● Phosphorothioate ● 2’F-RNA ● 2′-O-Methyl ● MOE-RNA ● LNA	● Amino ● Biotin ● Thiol ● Digoxigenin ● Acrydite ● DBCO ● Azide	● MGB Probe ● BHQ Probe ● Dabcyl Probe ● TAMRA Probe

Symbol	Base Type	Standard Ratio	Price
Standard Level	Superior Level
R	A,G	50% : 50%	Quote	Quote
Y	C,T	50% : 50%
M	A,C	50% : 50%
K	G,T	50% : 50%
S	C,G	50% : 50%
W	A,T	50% : 50%
H	A,C,T	33% : 33% : 33%
B	C,G,T	33% : 33% : 33%
V	A,C,G	33% : 33% : 33%
D	A,G,T	33% : 33% : 33%
N	A,C,G,T	25% : 25% : 25% : 25%

Length	Quantity	Purification	Turnaround TIme	Price
90-109 mer	1 OD	PAGE / Dual-PAGE	5-6 business days	Quote
110-129 mer	1 OD	5-7 business days
130-150 mer	0.5 OD	5-8 business days

QUOTE

Deliverables

Lyophilized dry DNA oligo powder, 1 tube (Transparent or dark) or 96-well and 384-well plates.

Certificate of Analysis (COA): including sequence information, OD, Tm, etc.

FAQs

How do you determine how many nmol values of oligos need to be synthesized?

It is determined according to the purpose of the experiment. Take about 20 bases oligos as an example: for routine PCR amplification, you can choose 5nmol (about 1OD), which can be used to do 200 times of 50μl standard PCR reaction; for gene splicing or ligation after annealing, you can choose 5nmol (about 1OD).

How is Oligo quantified?

The absorbance of the solution is quantified by UV spectrophotometer at 260 nm. After the DNA dry powder is dissolved in water, a portion of the solution is taken and diluted to 1 ml. After this, the absorbance is measured, which is the OD value of the measured volume. Through this method, the OD value of the mother liquor can be calculated.

How to store DNA oligo？

At -20°C, dry DNA oligo powder are stable for at least 1 year and oligo solutions for more than 6 months.

More FAQs>>

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