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In Vitro Transcription

The General Introduction of

In Vitro Transcription

In vitro transcription takes linear DNA sequences as a template, using T7, T3, or SP6 RNA polymerase to synthesize RNA from the DNA template sequence.

Synbio Technologies offers efficient and cost-effective in vitro transcription RNA synthesis through our Syno synthesis platform. Our customers can provide us with the DNA templates, such as plasmid and/or PCR products, or we can conduct the de novo gene synthesis of the target DNA sequences. After codon optimization, 5‘-capping, 3’-addition and nucleoside modification, the transcribed RNA is suitable for gene editing, gene function research, gene diagnosis, structural research and nucleic acid vaccine development.

Highlights

One-Stop Service from DNA Template Synthesis to IVT RNA
Fully Customized Synthesis
Guaranteed Service: No RNA, No Pay
Free Codon Optimization
> 95% Purity & Strict QC
Abundant RNA Types

Service Details

Size/Scale	PolyA Tails	Cap	Modifications	Purifications	Turnaround Time	Price
40-9000 nt 2 µg-g	• Customized tail • 100-150A tail (enzymatic method) • 120A tail • No tail	• One stop capping (Clean cap AG, ARCA cap, m7GpppG Cap) • Enzymatic capping (Cap 0, 1) • No cap	• 5-mUTP • 5-moUTP • ψ-UTP • N1-Me-ψ-UTP • Cy3, Cy5, AF488 • Unmodified	• Silica column purification • LiCl purification • Phenol/chloroform extraction • HPLC	IVT: 5-10 BD Gene Synthesis: 10-15 BD	2 µg start from $200 1 mg start from $1,000

* The price only refers to the transcription, gene synthesis price and modification will be charged separately.

Quality Control

QC Items*	Method	Specifications	Default QC	QC Price
Appearance	Visual inspection	Clear, colorless, free from visible particulates	√	Free
A 260/280 Ratio	UV Absorbance	1.70 - 2.30	√	Free
Quantity	UV Absorbance	Quantity is ± 5%	√	Free
RNA size & purity*	Denaturing agarose gel electrophoresis	Detect expected size band	√	Free
Endotoxin	TAL assay	< 10 EU/mg	X	$50 per reaction
DNA template residue test	PCR & Gel electrophoresis	PCR product from the RNA products is <5% compared to positive control	X	$125 per reaction
Bioburden	Streak inoculation	No growth after 48 hours	X	$50 per reaction
Mycoplasma	Mycoplasma PCR Detection	No mycoplasma is detected in the RNA products	X	$125 per reaction
RNA Sequence confirm	RT-PCR & Sanger sequencing	Sequencing results are consistent with the confirmed RNA sequence	X	Starting at $250
Functional test	Transfection & Western Blot	Detect expected size protein	X	Quote

* For more QC options, please contact us at quote@synbio-tech.com.

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Workflow

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Templates For In Vitro Transcription

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dsDNA templates often increase transcription yield of the RNA. Synbio Technologies provides multiple types of DNA templates for in vitro transcription, including the linearized DNA plasmids, PCR products, synthetic oligos, and cDNA.

Deliverables

Liquid RNA

COA

Lyophilized template plasmid

Case Study

RNA (1010 nt) on denaturing agarose gel. The RNA was synthesized by in vitro transcription and purified via LiCl purification method.

FAQs

How do I ensure that the synthesized mRNA has high purity?

High purity mRNA can be obtained by optimizing in vitro transcription conditions and using efficient purification methods (e.g. column chromatography, gel electrophoresis, etc.).

Why is 5‘ capping and 3’ tailing required for mRNA synthesis?

5‘ capping and 3’ tailing help to improve the stability and translation efficiency of mRNA and prevent it from being degraded inside the cell.

How should I store my RNA products?

mRNA can remain stable for at least one year if stored at -80°C and not subjected to repeated freeze-thaw cycles, though stability varies with mRNA length. For long-term storage, keep mRNA at -80°C; for short-term, -20°C is recommended.

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