The General Introduction of Insect Expression
Proteins expressed using the baculovirus-insect expression system retain full biological functionality and can accommodate larger exogenous fragments. This makes them ideal for expressing large DNA segments.
Synbio Technologies' experienced technical team has developed a baculovirus-insect cell expression and purification platform that supports production scales of up to 500L. This platform produces recombinant proteins with over 95% purity. Our team excels in expressing a range of challenging proteins including toxic proteins, kinases, large proteins, membrane proteins, and intracellular proteins.
Highlights
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- Free Codon Optimization
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- One-stop Service from Genes to Proteins
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- Multiple Purification Methods to Meet Different Requirements
Service Details
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Service Steps
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Description
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Turnaround Time
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Price
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Gene Synthesis (optional)
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• Codon optimization
• Gene synthesis
• Vector construction
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Starting from 10 BD
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Quote
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Baculovirus preparation
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• Generation of recombinant Bacmid DNA
• Generation of P1 stock, P2 stock and determination of virus titer
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3-5 weeks
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Starting from $2,699
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Validation and analysis of protein expression
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• P2 generation virus infect SF9 cells
• Expression analysis validation (WB)
• Ni column affinity purification
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2-4 weeks
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Protein Expression and Purification
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• 1 L or larger scale expression
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1-2 weeks
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* The delivery time of this step may vary due to the length and/or difficulties of the sequence(s). Please contact our project manager for a quote.
Deliverables
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2-5 μg lyophilized plasmid DNA
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1 tube of glycerol bacteria/puncture bacteria
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Sequencing validation report
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Experiment report
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A small amount of Protein
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Expression evaluation report
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Purified protein products
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Certificate of analysis (COA)
FAQs
The most commonly used insect cell lines are Sf9, Sf21, and High Five (T. ni) cells, derived from the fall armyworm and cabbage looper.
Recombinant proteins produced using this system is commonly used in research, pharmaceuticals, vaccine production, and biotechnology.
The baculovirus-insect cell expression system is a method used to produce recombinant proteins in insect cells using baculovirus as a vector. This system is known for its ability to express proteins with post-translational modifications similar to those in higher eukaryotes.
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