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The General Introduction of Gene Shuttle Vector

Synbio Technologies is excited to introduce our gene shuttle vector related services! Our modified shuttle vector enables protein expression detection without requiring transformation operations.

Compared to existing expression vectors in the market, our shuttle expression vector offers enhanced efficiency and reliability. The vector streamlines experimental procedures, which significantly reduces complexity. Additionally, it’s high-efficiency dual-system compatibility enhances expression capabilities. When used in combination with our ProXpress rapid protein detection kit, you can preview the expression of synthesized plasmids before receiving them - accelerating the progress of your scientific research!

Highlights

Highly efficient and stable protein expression in eukaryotic and prokaryotic systems.
No need to transform expression strains such as BL21 (DE3), shortening the verification cycle.
Instant protein expression detection with ProXpress.
Customized vector transformations available to meet specific research needs.

Service Details

Service Type	Gene Length	Price	Turnaround Time (Business Days)
GeneShuttleVector	<251 bp	$50	5-10
	251-1,500 bp	$0.20/bp	5-10
	1,501-3,000 bp	$0.21/bp	10-15
	3,001-4,500 bp	Quote	15-20
	4,501-6,000 bp	Quote	20-25
	6,001-8,000 bp	Quote	Quote
	>8,000 bp	Quote	Quote

* Turnaround time applies to gene synthesis of non-complex sequences. For gene synthesis of complex sequences, please consult: quote@synbio-tech.com.

** Optional protein validation with ProXpress for $35 each.

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Workflow

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Deliverables

2~5 µg lyophilized plasmid DNA

Sequencing chromatogram

Certificate of analysis (COA)

ProXpress validation reports

Case Study

1. Background

The shuttle vector expressing green fluorescent protein (GFP) wastransferred into prokaryotic cells ( Mach1-T1 ).

The results show that

The supernatant of the bacterial lysate was observed under ultraviolet (UV) light and green fluorescence was detected.

Using the Flag-tag protein rapid detection card,protein expression was confirmed10 minutes later,showing high levels of proteinexpression.

2. Background

Aconstructed shuttle expression vector was transfected into HEK 293 cell and compared witha conventional expression vector.

The results show that

Comparedto the conventional vector, the modified shuttle expression vectorshowed significantlyhigherproteinexpression in HEK 293 cell.

FAQs

What are the possible reasons for failed gene cloning when inserting a target gene into a shuttle vector?

Possible causes include:

Unreasonable primer design
Poor template quality
Incorrect PCR program settings.

Compared to conventional vectors, what advantages do shuttle vectors offer?

Dual system compatibility: Shuttle vectors contain two or more replicons from different genetic backgrounds,enabling replication in two or more different organisms.
Selective markers: Shuttle vectors usually contain selective markers, such as antibiotic resistance genes, allowing for easy screening of clones containing the target gene via antibiotic selection.
Higher Efficiency: Shuttle vectors are typically more efficient when introduced into the target biological system.

What are the application areas of shuttle vectors?

Shuttle vectors are used in various fields, including:

Gene function research
Protein interaction analysis
Drug target screening
Vaccine development

The efficiency of shuttle vectors makes them suitable for various application, ranging from academic research to drug development,supporting scientists and researchers in their pursuit of research and innovation.

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