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Monoclonal Antibody Preparation
Maximize Your Research Potential with the Widest Range of Antibody Clones!
Quote
Home > Antibody Services > Monoclonal Antibody Preparation
The General Introduction of Monoclonal Antibody Preparation
Monoclonal antibody technology involves fusing a single B lymphocyte capable of producing antibodies with myeloma cells, resulting in hybrid cells that can generate both antibodies and proliferate indefinitely, thereby enhancing antibody production. This technology has been widely applied in fundamental research, medical diagnosis, and tumor-targeted therapy, among other fields.
Synbio Technologies has a robust antibody drug discovery platform utilized by our talented scientific team. Our platform provides a full range of services in custom monoclonal antibody production including immunization, hybridoma development, antibody discovery, antibody functional screening assays, antibody engineering, and antibody expression. Using this platform, we can deliver up to 10 high-quality functional hybridoma clones to jumpstart our customers’ antibody research.
Highlights
  • Proprietary Immunization Technology
    Universal Antigen can induce antibodies to recognize epitopes containing less than 5 aa
  • One-Stop Solution
    Streamlined strategy focuses on immunization, hybridoma generation, and characterization
  • High-Throughput Screening
    Immunization and high-throughput screening is optimized for affinity measurements, epitope binning, isotyping, etc.
  • Comprehensive Functional Assay
    Extensive experience held in antibody affinity ranking, affinity measurement, cross-reactivity, and other in-vitro biological analyses
Service Details
Milestones Details TAT Deliverable
Animal Immunization Blood collection before immunization 7-9 weeks Interim report (Including antiserum titer test results)
First immunization
(Antigen plus Freund's complete adjuvant)
Second immunization
(Antigen plus Freund's incomplete adjuvant)
Third immunization
(Antigen plus Freund's incomplete adjuvant)
Blood sampling and ELISA test
Fourth Immunization (Antigen is dissolved in PBS or saline)
Animal blood collection

Cell fusion and screening 		Cell fusion and screening 5-6 weeks Interim report (Including screening data)
Subcloning and cell expansion
Cell line cryopreservation
Antibody production and purification Hybridoma cell culture 4-6 weeks 1-3mg monoclonal antibody with a titer of 100,000
Final experiment report
Antibody purification
ELISA, SDS-PAGE, concentration determination
QUOTE
FAQs
What are the differences between monoclonal antibodies and polyclonal antibodies?

    Monoclonal Antibody Polyclonal Antibody
    Identify a unique epitope of a specific antigen Identify multiple epitopes of a specific antigen
    Specific detection of an unique target epitope, without cross-reactions with other antigens; lower staining background interference More cross-reactions with similar antigens; can amplify the signal of the target protein with low expression level; higher staining background interference
    Detection of an unique antigen or a single member of a protein family More tolerant to small antigen changes and can detect homologous protein families
    Impure immunogens can also produce high purity antibodies Higher purity of the immunogen will obtain better polyclonal antibodies
    Easy to standardize; the antibody test results from different batches are stable and consistent The quality of antibodies varies greatly from batch to batch
    Higher production cost; longer turnaround time Lower production cost; shorter turnaround time

How to choose mAb or pAb?

    Monoclonal antibody uses: 

    ● Quantification of protein expression level 

    ● Immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) 

    ● Detection of structural changes and of antigens' phosphorylation states 

    Polyclonal antibody uses: 

    ● Capture of as many antigens as possible (e.g. IP or CHIP) 

    ● Detection of denatured proteins 

    ● Detection of proteins which may have undergone glycosylation or morphologic changes

What are the advantages and disadvantages of monoclonal antibodies ?

    Advantages：

    • Highly specific recognition of only one epitope of an antigen

    • hybridoma cell lines have the ability to produce unlimited quantities of antibodies

    • High consistency

    • Minimal background noise and cross-reactivity

    • Excellent for affinity purification

    Disadvantages：

    • More expensive

    • Longer production time

    • Possible lower signal

    •  More vulnerable to loss of epitope through chemical treatment of the antigen. This can be offset by pooling two or more monoclonal antibodies

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