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The General Introduction of PCR Cloning & Subcloning

PCR cloning and subcloning, two common recombination procedures are widely used in various important applications. Our PCR cloning services offer a quick way to clone genes that does not rely on the vector's enzymatic sites, allowing for direct cloning of target gene fragments into the target sites of any vector. The PCR subcloning service employs existing enzyme sites to move DNA fragments from one vector to another.

Why Choose Synbio Technologies?

Synbio Technologies integrates DNA synthesis equipment and cloning technology to provide comprehensive PCR cloning and subcloning services. Our custom DNA cloning solutions can clone the target gene into any desired location of the vector, meeting your specific design requirements.

We also offer effective DNA subcloning services to ensure that your downstream tests go smoothly, we are your go-to source for dependable DNA cloning services and advanced PCR cloning technology.

Highlights

Fast Turnaround Time
100% Sequence Accuracy
Independent of Vector Restriction Sites
Professional Technical Team

Service Details

Service Type	Fragment Length	Turnaround Time (Business Days)	Price
PCR Cloning & Subcloning	<2 kb	5-10	Starting from $75
	2-3 kb	5-10
	>3 kb	Quote

* The turnaround time applies to non-complex sequences. Please contact quote@synbio-tech.com to quote for the complex sequences.

* Note

DNA Sequence

If the sequence has not been verified, we will provide Sanger sequencing verification. (Additional fees apply)

Specific Vectors

1. A Full-length sequence is required.

2. If there is no sequence information, we will provide Sanger sequencing verification. (Additional fees apply)

Rare RE or Special Molecular Biological Reagents

1. Customers will provide these reagents.

2. Synbio Technologies will purchase them for you (Additional fees apply) and return the excess after the delivery.

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Workflow

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Specific Applications

PCR cloning and subcloning technology has a wide range of applications, including genetic engineering, agricultural production, biopharmaceuticals, and more. Click Introduction to Molecular Cloning: Techniques and Applications to learn more.

• Genetic Engineering

In genetic engineering, gene function research uses PCR cloning and subcloning services to clone specific genes, thereby studying their functions and regulatory mechanisms in cells. Gene editing uses PCR cloning technology to insert edited gene fragments into the target genome for gene knockout or knock-in experiments.

• Agricultural Production

Crop improvement combines PCR cloning technology and subcloning services to insert disease-resistant, insect-resistant, or stress-resistant genes into crops, increasing crop yield and quality. In the creation of biopesticides, DNA cloning services are used to clone insect-resistant proteins from plant or microbial sources in order to create new biopesticides that lessen reliance on chemicals.

• Biopharmaceuticals

In biopharmaceuticals, recombinant protein manufacturing is accomplished via PCR cloning and subcloning technology, which inserts the gene encoding the therapeutic protein into the expression vector to make recombinant protein drugs such as insulin and monoclonal antibodies.

Subcloning services are used in vaccine research to clone pathogen-specific antigen genes to create and produce vaccines that improve immune response. Furthermore, gene therapy employs custom DNA cloning techniques to clone therapeutic genes into viral vectors for gene therapy research and treatment of genetic diseases.

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Deliverables

Lyophilized plasmid DNA (2~5 µg)

Sequencing results in .ab1 format

Certificate of Analysis (COA)

FAQs

What is PCR subcloning service?

The PCR subcloning service is a molecular biology service based on the Polymerase Chain Reaction (PCR) technology. It is utilized to amplify specific DNA fragments from complex DNA samples and subsequently clone them into vectors, facilitating further genetic engineering or research.

What are the applications of PCR cloning service?

PCR cloning services have extensive applications in the field of molecular biology, including gene cloning, gene expression analysis, gene mutation detection, genetic disease diagnosis, and more. Leveraging the PCR cloning technology, researchers can efficiently amplify and clone target DNA fragments, providing robust support for genetic research and applications.

How do I improve the success rate of PCR subcloning?

• Optimize PCR Conditions: Ensure high purity and concentration of the target DNA fragments obtained through PCR amplification.

• Select an Appropriate Vector: Choose the suitable vector type and cloning sites based on the experimental requirements.

• Strictly Control Experimental Conditions: Rigorously manage factors such as temperature and time during the ligation and transformation processes.

• Conduct Multiple Rounds of Screening and Verification: Obtain positive clones through multiple rounds of screening and identification, followed by sequencing validation.

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