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The General Introduction of Bacterial Expression

Synbio Technologies provides a comprehensive solution that covers every step from codon optimization and gene synthesis to recombinant protein expression and purification, all tailored to your experimental needs. We also offer additional services like tag removal.

With our extensive experience and advanced E.coli expression system, we guarantee high-quality and pure protein production within a minimum of 4 weeks TAT, ensuring you have what you need for your downstream experiments timely.

Highlights

Free Codon Optimization
TAT as Quick as 4 Weeks
Competitive Price, Starting from $1299
Customizable One-stop Service
Quality Guaranteed, No Target Protein, No Charge

Service Details

Steps	Service Details	Turnaround Time	Price
Gene synthesis	• Codon optimization and gene synthesis • Sub cloning into appropriate expression vector • Various tags are available, such as His, SUMO,TRX,GST…	≤2 weeks	Starting from $1299
Protein Expression Testing and Purification	• Transform plasmids into appropriate bacterial expression strain • Protein expression evaluation	≤3 week
Protein Expression Testing and Purification	• Scale up protein expression • Purification to reach desired protein amounts and purity • Tag removal and separation of tag-free protein (optional) • Refolding in case of insoluble protein (optional)	≤3 week
QC & Delivery	• SDS-PAGE • Western Blot (optional) • Bradford assay for quantitation • Protein delivery	≤1 week

* Membrane proteins and toxic proteins are excluded
* For gram level large-scale protein production, please contact quote@synbio-tech.com

Catalog	Description
HX-85Pro	85% purity, tagged protein, protein MW between 15KD and 120KD
HX-90Pro	90% purity, tagged protein, protein MW between 15KD and 120KD
HX-95Pro	95% purity, tagged protein, protein MW between 15KD and 120KD
HX-15Pro	85% or 90% purity, tagged protein, protein MW ≤ 15KD

* Evaluation is required before the project starts.

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Workflow

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Deliverables

2-5 µg lyophilized plasmid DNA

1- 5 mg protein products

Purity options: 85%, 90%, 95%

Endotoxin level options: ≤0.1 EU/μg

Certificate of analysis

Case Study

FAQs

What are the advantages of prokaryotic protein expression systems?

Prokaryotic protein expression systems (e.g. E. coli) have the advantage of being fast, high yield, and affordable; often suitable for large-scale protein production.

How to improve the expression level of soluble proteins?

You can improve the expression level of soluble proteins through several different methods, some being codon optimization, changing the expression host strain, and adjusting the expression conditions (e.g., temperature, medium components, etc.)

Why are proteins expressed in E. coli prone to form inclusion bodies?

In the E. coli expression system, some exogenous proteins may form inclusion bodies due to misfolding. This is mainly due to the complex structure or toxicity of the exogenous proteins.

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